Alignment and hydration profile of TIMs. (A) Interfacial residues in sequence alignment (according to the HsTIM dimer interface) and their hydration profiles. The color of the the letters indicates the number of contacting interfacial water molecules of the residue (#water). The 20 interfaces are grouped into three categories: 11 Eukarya, 6 Bacteria, and 3 Archaea. (B) shows the corresponding conservation score of HsTIM dimer interface residues according to Consurf. Residues' positions in (A) and (B) are aligned and they are divided into four subregions according to HsTIM dimer interface.