Skip to main content
Figure 2 | BMC Bioinformatics

Figure 2

From: Parallel comparison of Illumina RNA-Seq and Affymetrix microarray platforms on transcriptomic profiles generated from 5-aza-deoxy-cytidine treated HT-29 colon cancer cells and simulated datasets

Figure 2

EIV Regression Model Comparing Microarray and RNA-Seq Gene profiles. EIV regression model is constructed for independent variable (microarray normalized gene intensities in log2 unit-free scale) and dependent variable (RNA-Seq FPKM values in log2 unit-free scale) for each of the experimental groups (5 µM, 10 µM and 0 µM) of HT29 samples. Log2 scaled unit-free normalized gene intensities are shown as grey circles in the scatter plot and EIV regression line is drawn in bold black. For each of the plots, a dashed reference line of Y = X (corresponding to perfect platform agreement) is also included to indicate the deviation of the real regression line from the reference. The estimated regression equation is shown in the lower-right section of each plot. The 95% bootstrap confidence interval for the regression intercept and slope (α and ß) are shown on the top of each plot.

Back to article page

BMC Bioinformatics

ISSN: 1471-2105

Contact us