Comparison of the performance of methods with the Platinum Spike dataset. Platinum Spike dataset, Venn diagram of top 1940 genes by (A) the FCROS, RP, SAM and TREAT methods or (B) by the FCROS, FC, WAD and RP methods. Only genes that correspond to true DE genes are represented. M-A plot: (C) highly expressed genes where magenta diamonds are used for the 24 genes detected by the TREAT method (panel A), black triangles are used for the 15 genes detected by the SAM and TREAT methods (panel A), cyan circles are used for the 71 genes detected by the WAD method (panel B); and (D) weakly expressed genes where green squares are used for the 54 genes detected by the FCROS, FC and RP methods (panel B). Additional file 1: Figure S4 shows the full M-A plot of this dataset.