From: Quantitative group testing-based overlapping pool sequencing to identify rare variant carriers
Sequencing region (Mb) | Sample size | Frequency of variant | # of pools | Data throughput (Gb) | Cost saving | |
---|---|---|---|---|---|---|
Haploid sample | 5 | 200 | 0.5% | 20 | 83.4 | 85.7% |
5 | 200 | 1% | 30 | 124.8 | 78.6% | |
5 | 200 | 1.5% | 40 | 128.8 | 73.4% | |
Diploid sample | 30 | 200 | 0.25% | 30 | 669.6 | 53.4% |
30 | 100 | 0.5% | 20 | 292.8 | 52.6% | |
30 | 100 | 1% | 30 | 534.6 | 20.3% |