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Table 2 Most cost-effective designs for different scenarios

From: Quantitative group testing-based overlapping pool sequencing to identify rare variant carriers

  Sequencing region (Mb) Sample size Frequency of variant # of pools Data throughput (Gb) Cost saving
Haploid sample 5 200 0.5% 20 83.4 85.7%
5 200 1% 30 124.8 78.6%
5 200 1.5% 40 128.8 73.4%
Diploid sample 30 200 0.25% 30 669.6 53.4%
  30 100 0.5% 20 292.8 52.6%
  30 100 1% 30 534.6 20.3%
  1. The sequencing region for haploid samples was set as 5 Mb to fit the average length of the bacterial genome. The sequencing region for diploid samples was set as 30 Mb to fit the human exome sequencing.