Skip to main content

Table 1 Cost of sequencing to achieve a given level of heterozygous SNV detection sensitivity

From: Variant detection sensitivity and biases in whole genome and exome sequencing

Method Lane usage Mean on-target depth Sensitivity Cost
Whole genome 1 lane/sample 11X 94.0% 4.60
2 lanes/sample 22X 98.5% 8.79
Exome 16 samples/lane 22X 91.1% 1.00
12 samples/lane 29X 93.4% 1.09
8 samples/lane 44X 95.9% 1.28
6 samples/lane 58X 96.9% 1.46
4 samples/lane 88X 98.1% 1.63
  1. All costs have been normalised against the cheapest exome sequencing (16 samples per lane). Estimated costs include library preparation, exome capture and multiplexing where applicable, and paired-end sequencing on Illumina HiSeq.