Alignment and network of orthology of the Apc13 family. (a) Multiple sequence alignment of some Apc13 orthologs, including those from Candida glabrata and Oryza sativa. Conserved positions across all shown species are highlighted in bright yellow, those that are conserved in five out of the seven sequences are highlighted in dark yellow. Species abbreviations and accession numbers are listed in Additional file 2: Table S14. (b) The network of orthology for the Apc13 family displayed in Cytoscape. There are three tightly connected clusters representing the metazoan and two fungal groups. The false positive predictions are clearly separated from the interconnected clusters (grey nodes). Nodes are scaled according to E-value with low E-values having large circles and high E-values having small ones. An edge-weighted spring-embedded layout was chosen.