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Table 2 Width of a replicate based confidence interval

From: Impact of variance components on reliability of absolute quantification using digital PCR

Replicates (m) Width Improvement
2 1.000  
3 0.276 72.35%
4 0.177 35.94%
5 0.138 21.97%
6 0.117 15.48%
7 0.103 11.87%
8 0.093 9.60%
9 0.086 8.06%
10 0.080 6.94%
11 0.075 6.09%
12 0.071 5.42%
  1. The relative width of a confidence interval (proportional to t / m ) for a constant standard deviation is a function of the number of technical replicates and the t-quantile. The rightmost column gives the improvement (percentage decrease in width) when increasing the number of technical replicates with one. Note, that in this table we only consider uncertainty due to technical variability and that reducing technical variability does not eliminate biological variability. Hence, in experiments for comparing nucleic acid content across biological conditions an appropriate number of biological repeats, each with technical replicates, will always be required.