Heat map of t-values obtained from the linear regression analysis of dependence between the inferred TF-binding affinities and the measured TF tag counts in ChIP-seq experiment. For 5 mouse TFs with available in vivo ChIP-seq data sets, the relationships between the predicted TF-binding affinities by using PBEM from in vitro data and the measured TF occupancy data from in vivo ChIP-seq experiments were investigated by the linear regression model, respectively. In the figure, t-value represents the significance of such correlations. The top 500, 1000, 2000, and all called peaks were considered in the linear regression analysis, respectively. Here, the top-ranked peaks were sorted in descending order by the number of tags found at the peak.