Table 2 Output format for forward primers
From: A tool for design of primers for microRNA-specific quantitative RT-qPCR
ssc-let-7a | tgaggtagtaggttgtatagtt | Â | Â | Â | Â | Â | Â | Â | Â |
|---|---|---|---|---|---|---|---|---|---|
Primers | Â | Â | Â | Â | Â | Â | Â | Â | Â |
Name | Seq | Length | score | two_last_score | three_last_score | five_last_score | three_self_anneal | internal_self_anneal | five_prime_score |
F_1 | gcagtgaggtagtaggttgt | 16 | 0.64 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 10 |
F_2 | gcagtgaggtagtaggttg | 15 | 0.56 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 10 |
F_3 | cgcagtgaggtagtaggt | 13 | 0.42 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 10 |
F_4 | cgcagtgaggtagtaggtt | 14 | 0.34 | 0.7 | 1.0 | 1.0 | 1.0 | 1.0 | 10 |
F_5 | gcagtgaggtagtaggttgta | 17 | 0.25 | 0.7 | 1.0 | 0.5 | 1.0 | 1.0 | 10 |
F_6 | cgcagtgaggtagtagg | 12 | 0.25 | 0.7 | 1.0 | 1.0 | 1.0 | 1.0 | 10 |
F_7 | gcagtgaggtagtaggttgtat | 18 | 0.09 | 0.7 | 0.3 | 0.5 | 1.0 | 1.0 | 10 |
F_8 | gcagtgaggtagtaggttgtata | 19 | 0.02 | 0.7 | 0.3 | 0.5 | 0.2 | 1.0 | 10 |