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Table 2 Output format for forward primers

From: A tool for design of primers for microRNA-specific quantitative RT-qPCR

ssc-let-7a tgaggtagtaggttgtatagtt         
Primers          
Name Seq Length score two_last_score three_last_score five_last_score three_self_anneal internal_self_anneal five_prime_score
F_1 gcagtgaggtagtaggttgt 16 0.64 1.0 1.0 1.0 1.0 1.0 10
F_2 gcagtgaggtagtaggttg 15 0.56 1.0 1.0 1.0 1.0 1.0 10
F_3 cgcagtgaggtagtaggt 13 0.42 1.0 1.0 1.0 1.0 1.0 10
F_4 cgcagtgaggtagtaggtt 14 0.34 0.7 1.0 1.0 1.0 1.0 10
F_5 gcagtgaggtagtaggttgta 17 0.25 0.7 1.0 0.5 1.0 1.0 10
F_6 cgcagtgaggtagtagg 12 0.25 0.7 1.0 1.0 1.0 1.0 10
F_7 gcagtgaggtagtaggttgtat 18 0.09 0.7 0.3 0.5 1.0 1.0 10
F_8 gcagtgaggtagtaggttgtata 19 0.02 0.7 0.3 0.5 0.2 1.0 10