Table 4 Success rate of primer design
From: A tool for design of primers for microRNA-specific quantitative RT-qPCR
Validated assays | Primers designed by miRprimer | 1stdesign successful | ||
|---|---|---|---|---|
F primer | R primer | |||
16 | 16 | 16 | 16 | 100% |
41 | 41 | nra | 39 | 95% |
38 | nra | 38 | 37 | 97% |
84 | 80 | 81 | 78 | 93% |