Figure 1

CIG-P circular diagram. On the outer circle, all protein reference sets are placed, separated by color. The width of the colored sections is proportional to the number of proteins they encompass. As scale, the white sections have a width of 3 proteins. A) AP-MS data displayed in circular layout in cytoscape. B) The AP-MS dataset is projected onto a functionally annotated protein dataset. The interactors of SRPK1 were drawn in colored arcs while the kinase substrate data was drawn in black arcs. As seen, the interactors and kinase substrates of SRPK1 are specific to sub-complexes of the spliceosome (see main text for discussion). C) Lenticular function CIG-P diagram (reappearance filtering ON) comparing the interactors of four kinases to interactors of SRPK1. From the black section of SRPK1 (bait protein), colored arcs are drawn representing the high quality protein-protein interactions found in each AP-MS experiment. As seen from the diagram, interactors of SRPK1 and SRPK2 are similar, while SRPK3 and PRPF4B show distinct differences in their interactome.