Statistical analysis of differential gene expression relative to a fold change threshold on NanoString data of mouse odorant receptor genes

Table 1 Area under the ROC curve (AUC) for various methods on simulated data

AUC results completing Figure1
*Simulated FC between test and control = 1.3*
		1% DE genes	10% DE genes	20% DE genes
TREAT, FC = 1.3	AUC* [95%PI]	96.1 [95.6-96.7]	96.3 [96.2-96.5]	96.4 [96.3-96.6]
tTREAT, FC = 1.3	AUC* [95%PI]	95.9 [95.3-96.4]	96.2 [96.1-96.3]	96.2 [96.1-96.3]
*Simulated FC between test and control = 1.5*
	1% DE genes	10% DE genes	20% DE genes
TREAT, FC = 1.5	AUC* [95%PI]	97.3 [96.8-97.7]	97.3 [97.2-97.5]	97.3 [97.2-97.4]
tTREAT, FC = 1.5	AUC* [95%PI]	97.0 [96.5-97.5]	97.0 [96.8-97.1]	96.9 [96.8-97.0]
*Simulated FC between test and control = 2*
		1% DE genes	10% DE genes	20% DE genes
TREAT, FC = 2	AUC* [95%PI]	98.0 [97.7-98.4]	98.2 [98.1-98.3]	98.2 [98.1-98.3]
tTREAT, FC = 2	AUC* [95%PI]	97.3 [96.8-97.8]	97.5 [97.3-97.6]	97.5 [97.3-97.6]
AUC results completing Figure 2
*Simulated FC between tets and control = 1.5 and Stringent test*
		1% DE genes	10% DE genes	20% DE genes
tTREAT, FC = 1.5	AUC* [95%PI]	96.7 [96.2-97.2]	97.0 [96.9-97.2]	97.0 [96.8-97.1]
tTREAT, FC = 2.5	AUC* [95%PI]	91.1 [89.5-92.7]	93.7 [93.5-94.0]	93.7 [93.5-94.0]
tTREAT2, FC = 2.5/1.5	AUC* [95%PI]	96.7 [96.1-97.3]	97.3 [97.1-97.4]	97.2 [97.1-97.4]
AUC results completing Figure 3
*Simulated FC between test and control = 2.5 and non-stringent test*
		1% DE genes	10% DE genes	20% DE genes
tTREAT, FC = 2.5	AUC* [95%PI]	97.6 [97.2-98.1]	97.6 [97.4-97.7]	97.6 [97.5-97.7]
tTREAT, FC = 1.5	AUC* [95%PI]	97.7 [97.4-98.0]	97.7 [97.6-97.8]	97.8 [97.7-97.8]
tTREAT2, FC = 2.5/1.5	AUC* [95%PI]	96.7 [96.3-97.1]	96.7 [96.6-96.9]	96.7 [96.6-96.8]

ISSN: 1471-2105