Figure 1

DNAVaxDB workflow and system design. Data, including vaccine information and evidence from peer-reviewed publications, is manually curated into DNAVaxDB. Gene IDs, Protein IDs, or Nucleotide IDs are retrieved from NCBI and used by an internal script to gather detailed information about each protective antigen, which is then stored in DNAVaxDB. DNA and protein sequences can be used for BLAST, COG, and Vaxign analyses, and the latter two are stored in DNAVaxDB. A PubMed ID (PMID) from the relevant paper is also used by an internal script to gather comprehensive citation information for each study. Each vaccine and plasmid is assigned a Vaccine Ontology (VO) ID.