Reconstruction of PDZ domain shuffling in the Carma subfamily. Insertion of the PDZdomains from MPP4and Carma1into the ancestral Carma3. The two copies in Carma3subsequently experienced reciprocal loss - one domain was lost in the mammal ancestor and the other was lost in chicken. Carma2also experienced a gene loss in chicken. The ancestral architectures and domain insertions superimposed on the gene tree were reconstructed manually using events and associations of the ancestral nodes in the reconciled domain, gene, and species trees.