Obtaining long 16S rDNA sequences using multiple primers and its application on dioxin-containing samples

BMC Bioinformatics

Table 1 Statistics of reads, genera, and contig lengths of ten samples.

Sample	Raw reads	Trimmed reads	Confident reads	Total genus	Confident genus	No. of contigs	lcl/mrl. ≥1.5	lcl/mrl ≥2	lc identity ≥97%
S1	31,035	29,170	27,423	8	4	4	4	3	4
S2	38,100	35,007	30,242	144	28	23	15	6	11
S3	39,881	35,456	10,909	191	62	53	28	12	27
S4	91,44	4,590	478	65	9	9	4	3	3
S5	25,314	16,857	3,059	218	38	34	24	12	14
S6	14,709	8,347	854	117	18	13	5	2	2
S7	13,810	8,262	983	128	20	17	4	3	4
S8	11,379	9,214	2,145	230	47	37	18	5	18
S9	9,924	8,277	1,374	193	33	30	13	5	16
S10	12,084	8,216	1,685	124	23	20	11	4	7

A confident read is one with a ≥80 classification score. A genus is counted when there is a confident read. A confident genus is one with ≥10 confident reads. Confident reads of each confident genus were assembled and the longest contig (lc) analyzed. The last column shows the number of longest contigs with a ≥97% alignment identity to the 16S rDNA references. Abbreviations: longest contig length (lcl), mean read length (mrl).

ISSN: 1471-2105