From: Alternative splicing detection workflow needs a careful combination of sample prep and bioinformatics analysis
RNA selection
RNA concentration (ng)
Poly A +
rRNA depletion
0.5
2
100
1000
1500
NuGEN v2
nu05
nu2
nu100
ScriptSeq v1
✓
ss
TruSeq unstranded
ts100
ts1000
TruSeq stranded
tss (PolyA+) tss_total (rRNA depl)