Figure 4
LAM-PCR workflow and the corresponding adLIMS automated process. The LAM-PCR procedure [10] is reported as diagram (A) in which from a selected sample, different linear amplifications may be processed followed then by a first and a second exponential PCR amplification. The procedure is developed in adLIMSstarting from the experiment set-up (B) in which a user fills the form with the data relative to the experiment such as "Name", number of LAM-PCRs (N-LAM), "LAM ID" and "date". Then adLIMScreates a number of entries as reported in the field "N-LAM", for the subsequent step "LAM-PCR linear" (C), that will be then processed and expanded in the "LAM-PCR 1st exp" step (D) with details such as "Name", "Plate barcode" and "Enzyme". The last step is the "LAM-PCR 2nd exp" (E), corresponding to the second exponential amplification, in which the user completes additional data of the experiment ("concentration" and "quality").