Figure 2From: FastGroup: A program to dereplicate libraries of 16S rDNA sequencesSchematic of bacterial 16S rDNA showing conserved and hypervariable regions. Detailed information about the primers and their superposition on the bacterial 16S rDNA can be found at http://rrna.uia.ac.be/primers/data/BS/sec_model_fw.html. Bact27F (5' AGA GTT TGA TCM TGG CTC AG 3') corresponds to positions 9–27 of the E. coli 16S rDNA and is similar to BSF8/20. Bact517 (5' ATT ACC GCG GCT GCT GG 3') corresponds to positions 517–534 of the E. coli 16S rDNA and is similar to BSF517/17. Bact1492R (5' TAC GGY TAC CTT GTT ACG ACT T 3') corresponds to positions 1492–1514 of the E. coli 16S rDNA. The approximate sites for hypervariable regions (V1-V3) are shown as shaded boxes.Back to article page