Reannotation of the CELO genome characterizes a set of previously unassigned open reading frames and points to novel modes of host interaction in avian adenoviruses

Washietl, Stefan; Eisenhaber, Frank

doi:10.1186/1471-2105-4-55

Table 1 Unique coding sequences in CELO and related avian adenoviruses

From: Reannotation of the CELO genome characterizes a set of previously unassigned open reading frames and points to novel modes of host interaction in avian adenoviruses

CELO-ORF^a	Region^b	Strand	Length (aa)	Homologous sequences^c	Comment
ORF-1	794–1330	forward	178	FadV-9-ORF_847–1338	functional dUTP pyrophosphatase [4], homologous to ORF-1 proteins of mastadenovirus E4 region [5]
ORF-2	1999–2829	forward	276	FadV-9-ORF_1950–2753
ORF-8	37391–38239	forward	282	FadV-9-ORF_{37859–38668} FadV-10-ORF_2147–2911	Gam1, antiapoptotic, induction of heat shock response, inactivation of histone deacetylase 1, pRb/E2F pathway [6–10]
ORF-9	40037–41002	forward	321	FadV-9-ORF_{43595–42660} CFA40-ORF_17739-16381 EST-ORF-9	amino terminus unclear, since transcript was shown to be spliced up to 40133 [13]
ORF-10	41002–41853	forward	283	no homologs	Translation start is likely to be at pos. 41113
ORF-11	41958–42365	forward	135	FadV-9-ORF_{41461–41853}
ORF-12	5412-4462	reverse	315	FadV-9-ORF_6190-5243	amino terminus extended by 106 residues
ORF-13	4568-3549	reverse	339	FadV-9-ORF_5058-4261
ORF-14	3503-2892	reverse	203	FadV-9-ORF_4180-3536 FadV-9-ORF_3412-2837	amino terminus extended by 43 residues
ORF-16	39705-39286	reverse	139	FadV-10-ORF_4550-4209
ORF-17	39256-38717	reverse	179	FadV-9-ORF_41096-40596
				CFA40-ORF_15112-14642
				FadV-10-ORF_4023-3574
ORF-18/19	(36144)-34238	reverse	635	FadV-9-ORF_36385-34220 CFA40-ORF_10653-8782 FadV-10-ORF_4992-5864 FadV-10-ORF_6050-7456	Correction of an obvious error in the genomic sequence combines ORF-18 and ORF-19 to one single ORF. The amino terminus was extended by the translation of a short leader sequence that was shown to be spliced ahead of the original ORF-18 [13].
ORF-20	33832-32892	reverse	313	FadV-9-ORF_33963-32986 CFA40-ORF_8466-7741	amino terminus extended by 42 residues
ORF-22	32429-31812	reverse	205	FadV-9-ORF_32502-31930 CFA40-ORF_3306-2729	involved in pRb/E2F pathway [10]
ORF_28115-27765	28115-27765	reverse	117	FadV-9-ORF_30192-29797 CFA40-ORF_4777-4478 FadV-10-ORF_1814-1637	New conserved ORF, located between fibre and pVIII gene
ORF_32895-32434	32895-32434	reverse	154	FadV-9-ORF_32985-32509 CFA40-ORF_7552-7262	new conserved ORF, is translation of the original ORF-21 in a different frame

^a ORF-numbering in accordance to Chiocca et al. [4]^b coordinates in the genomic sequence of CELO (acc.no. NC_001720) ^c ORFs are named by their coordinates in the following nucleic acid sequences: NC_000899 (FAdV-9), AF155911 (CFA40) and AF160185 (FAdV-10). FAdV-10-ORF_1814-1637 is derived from entry AF006739, CFA40-ORF_3306-2729 from entry U40587. EST-ORF-9 is the translation of an EST sequence (BM491231) of a chicken EST library.

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ISSN: 1471-2105

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