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Table 1 Illustration of the Ampsig format with example values

From: A format for databasing and comparison of AFLP fingerprint profiles

# Field Example Value Information
1 Technique AFLP Full abbreviation of type of DNA markers, such as AFLP, SSR, RAPD
2 Experiment EcoRI_ACA-MseI_CAC For VNTR, RAPD and SSR markers, primer sequences will be given in full. In this example, a six-nucleotide cutting enzyme EcoRI and a four-nucleotide cutting enzyme MseI were used to digest genomic DNA. PCR reaction was conducted with an EcoRI primer with selective nucleotides ACA, and a MseI primer with selective nucleotides CAC
3 Source D Sonia id0011 Biological source of DNA. The binomial system with abbreviation of genus followed by species name is used to designate biological species. It is followed by a four-digit ID number to identify individual samples. In this example, DNA was isolated from a plant belonging to genus Dendrobium, species Sonia, with a lab-assigned ID 0011
4 UnitSize 1 Unit size of DNA fragment to define the unit spacing. It can also be interpreted as the sequence resolution. In this example, the 1 indicates that the sequence spacing is 1 base pair.
5 StartSize 50 Size of the first DNA fragment (in base pairs)
6 EndSize 90 Size of the last DNA fragment. This field is redundant, and is kept mainly for proof-reading
7 Annotation AFLP profile for D. Sonia Annotation of the fingerprint (only if required)
8 Signature A..B..DC..B.D.....CC...A.....DDA.A....C..D..BB Alternatively, a system employing the symbols [4, 3, 2, 1] for peaks and [0] for peak-absence may also be used.