Sample output from GASP. (a) The first 100 columns of a typical GASP ancestral sequence prediction output. Sequence order matches the default vertical ordering in the tree files produced by GASP. (b) A rooted version of the input tree. Lengths of branches are those defined in the input file. (c) A new version of the input tree with nodes labelled and branch lengths recalculated based on ancestral sequence prediction. Note. Data is output in (a) fasta format and (b & c) Newick format but for visual clarity the file has been shown using (a) BioEdit  and (b & c) TreeExporer .