Figure 2From: SeqDoC: rapid SNP and mutation detection by direct comparison of DNA sequence chromatogramsDifference pattern from a heterozygous SNP. A multiple peak in the test sequence (bottom trace) is characterised by an increase in the value in one channel (in this case the A at position 195 in the test sequence), and typically a decrease in the original channel (G), therefore giving a bi-directional peak in the difference profile similar to a direct substitution.Back to article page