Figure 5From: SeqDoC: rapid SNP and mutation detection by direct comparison of DNA sequence chromatogramsEffects of sequence misalignment. A minor misalignment of test and reference sequences by Gap4 causes a cyclical pattern in the difference profile (fig 5a, bottom trace) which increases signal noise. The fine adjustment algorithm of SeqDoC ensures that sequences are properly aligned (fig 5b) and eliminates this noise signal.Back to article page