Figure 6

Missed heterozygous base call. Tracediff does not identify the heterozygous base at position 191 in the test sequence (upper trace, fig 6a), possibly because of the weak signal strength and noise in the difference profile. The normalisation and difference profile optimisation algorithms built into SeqDoC give a very strong signal to noise ratio for this change in the difference profile (fig 6b) and make the substitution obvious.