The superfamily distance graph of the ephrin superfamily The graph contains only those edges which represent E-values of at least the superfamily cutoff 5e-10. The width of an edge is according to its E-value, here ranging from 5e-10 (thinnest edge) to 3e-149 (thickest edge). The subclustering procedure first splits off nodes from the bottom right of the graph as single sequence clusters. These sequences are predicted proteins which are not yet confirmed as functioning by any experiment. The last accepted split in the graph results in the partitioning into the two major groups of ephrin type A (left) and type B (right) sequences as shown by the dashed line. Single sequence clusters are added to the ephrin type B family in the subsequent singleton adoption step.