Figure 2From: MPrime: efficient large scale multiple primer and oligonucleotide design for customized gene microarraysWeb interface and example of primer pair output. The initial inputs were for the rat genome with GenBank accession NM_053986, gene ACTB, and keyword "cancer". The default values for the primer size, GC%, melting temperature, product size, and GC clamp length were used. The user was able to refine these choices to include the four genic regions indicated in the results. A similar screen is produced when oligonucleotides are chosen as well (not shown).Back to article page