Table 2 Sample set of genes and resulting primer pairs using MPrime1.0. The top sequence in each primer pair is the forward primer, the second sequence is the reverse primer.
Gene Name | Product Size | Primer Pairs |
|---|---|---|
GAPDH | 400 | AATGTGTCCGTCGTGGATCT GGGTCTGGGATGGAAATTGT |
Actb | 400 | GGCGCTTTTGACTCAGGATT AGTTGGGGGGACAAAAAAAA |
Sdf4 | 400 | AGACCTGCCAACCACTCATC TGCTTGCCAAAAACTTCACT |
Ubb | 400 | TTCTGTGAGGGTGTGAGGGT TTTATCCTGGATCTTGGCCT |
Ywhaz | 400 | ACAATGTTCTTGGCCCATGT AGGAAGAGGAGGAGGAAGGA |
Hprt | 400 | TTATGCCGAGGATTTGGAAA AACCTTAACCATTTTGGGGC |
Tubd1 | 400 | TAAGATGCTGGGTGTCCGTA TAAGAGCTGGCTGTTGCTGA |
Tuba1 | 400 | GACCCTCGCCATGGTAAATA AATCCACACCAACCTCCTCA |
Ppicap | 400 | ACTCCCTCCCTCTTTCCCTG CAGCAGAGAAAAGCTCCACC |
Hspca | 400 | AGGAAACCCAGACCCAAGAC ACACCAAACTGGCCAATCAT |
Htf9c | 400 | TGTGAATTCCTGGTCGGAGT TCTTTCTCTGTCCCTCCTCC |