The front page of the GObar website. Selections are made for each step, and the list of genes is entered in the final step before launching the program. The pruning of the tree is controlled in step 4. A node can only be pruned if every node under it also satisfies the pruning condition. The pruning options are explained in the subsection pruning the tree. Very strict pruning might cause useful results to be thrown away, but can also highlight the best information in the dataset. In contrast, using a low stringency at this step, or no pruning, can cause too much information to be presented. Step 5 allows the nodes to be either annotated with GO ids (preferred for large trees) or definitions of GO terms. The GOTermBrowser link at the top of the page allows search for GOids using keywords or regular expressions such as "*NA*binding" to explore the GO tree neighborhood of the search term.