BSR rationale and scatter plot example. A. BLAST score ratio analysis (BSR) calculation demonstrating how the two coordinates for plotting in figures B and C are calculated. B. Locations of the peptide spot revels the similarity that the peptide has to the two Query genomes. Use of a 0.4 separator is based on ~30% amino acid identity over 30% of the length of the peptide . C. Sample data obtained from comparison of Chlaymidia caviae GPIC (GenBank Accession Number AE015925) to the proteomes of Chlamydia muridarum strain Nigg (GenBank Accession Number AE002160) and Chlamydia pneumoniae AR39 (GenBank Accession Number AE002161) . Each point in the figure represents a single peptide in Chlaymidia caviae GPIC This analysis reveals that while these organisms are very similar, C. caviae is more similar to C. pneumoniae AR39 than C. muridarum strain Nigg due the skew of peptides with a slope of greater than 1.