Ndt80 binds to MSE Variants Differently in vitro. Purified MBP-ndt80, 51–350 was incubated with 35 base-pair radio-labeled oligos containing a centralized MSE (underlined capital letters) (a) or single base MSE variants and analyzed by EMSA (b). Single base-pair variants three nucleotides 5' and two nucleotides 3' of the central MSE sequence were also tested. All EMSA bands were quantitated relative to the wild-type MSE where the percent of labeled wild-type MSE shifted by Ndt80p was designated 1. The same data was shown both in the graph and in the table.