Figure 8
From: Microarray scanner calibration curves: characteristics and implications
Effect of normalization on the reproducibility and accuracy of log ratios (simulated data). A: log ratio correlation of dye-swap pair after mean normalization; B: log ratio correlation of dye-swap pair after Lowess normalization; C: Correlation of averaged log ratios of dye-swap pairs after mean and Lowess normalization. For D-I, the X-axis represents the log standard ratios directly calculated from concentrations. The Y-axis is represented as follows: D and E: log ratios for dye-swap pair (after mean normalization); F: Averaged log ratios of dye-swap pair (after mean normalization); G and H: log ratios for dye-swap pair (after Lowess normalization); I: Averaged log ratios of dye-swap pair (after Lowess normalization). Spots colored in green are of lower average intensity, whereas spots colored in blue are of higher average intensity.