Figure 5
From: Evaluation of absolute quantitation by nonlinear regression in probe-based real-time PCR
Bias between the NLR and the CT method. Real-time RT-PCR data for ACTB, IL18 and TNFA on RNA extracts from human gastric mucosa – a graphical representation of the observed bias between NLR and CT methods (inter-method agreement or inter-assay variation) in all three assays tested in this study. The final estimated copy numbers were transformed by log10 before calculation of bias. For these methods to be freely interchangeable, the bias plots should form a cloud around the zero line.