Table 1 The primer and probe sequences used in TaqMan PCR
From: Evaluation of absolute quantitation by nonlinear regression in probe-based real-time PCR
Assay | Primer | Sequence |
|---|---|---|
ACTB | PubMed accession number: BC002409 | |
Forward | 5' TGCCGACAGGATGCAG AAG 3' | |
Reverse | 5' GCCGATCCACACG GAGTACT 3' | |
Probe | FAM 5' AGATCAAGATCATTGCTCCTCCTGAGCGC 3' TAMRA | |
TNFA | PubMed accession number: X01394 | |
Forward | 5' CACGCTCTTCTGCCTGCTG 3' | |
Reverse | 5' GATGATCTGACTGCCTGGGC 3' | |
Probe | FAM 5' CCAGAGGGAAGAGTTCCCCAGGGAC 3' TAMRA | |
IL18 | PubMed accession number: BC007461 | |
Forward | 5' ATCGCTTCCTCTCGCAACA 3' | |
Reverse | 5' CATTGCCACAAAGTTGATGCA 3' | |
Probe | FAM 5' CAGGAATAAAGATGGCTGCTGAACCAG 3' TAMRA |