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Table 5 Experimentally determined pK values of α-MSH for MC4 receptor mutants. Shown is the average pK ± SD determined in radioligand binding competition using [125I]NDP-MSH as radioligand. (The numbers of independent replicates are in brackets). The significance, p, is calculated using nonparametric Wilcoxon Rank Sum test [29] (see Methods for details), when compared with the wild-type MC4 receptor.

From: Prediction of indirect interactions in proteins

Name pKi (log(nM)) ± SD (Repeats) p
MC4 5.73 ± 0.32 (22) -
Q43E/P48D 6.19 ± 0.14 (11) 1.3E-4
I66V 5.98 ± 0.26 (12) 9.8E-3
A89S 5.69 ± 0.14 (14) 6.4E-1
I102A 5.56 ± 0.30 (12) 1.8E-1
I129T 6.02 ± 0.15 (11) 4.1E-3
A135S 6.00 ± 0.15 (10) 1.0E-2
L141G 6.08 ± 0.15 (9) 3.0E-3
I251L 5.71 ± 0.36 (10) 7.0E-1
Y268I 5.88 ± 0.20 (14) 3.8E-2
Q43E/P48D/I66V 6.30 ± 0.17 (11) 1.5E-5
I66V/L141G 6.03 ± 0.20 (11) 4.6E-3
I129T/A135S 6.47 ± 0.51 (10) 2.3E-5
L141G/Y268I 6.01 ± 0.27 (12) 9.8E-3
Q43E/P48D/I66V/I129T/A135S 6.52 ± 0.17 (11) 1.4E-7
Q43E/P48D/I66V/I129T/A135S/L141G/Y286I 6.49 ± 0.31 (9) 1.8E-5
N240G/M241L/I245V 6.10 ± 0.10 (5) 1.1E-2
V253I/V255F/V256L 6.19 ± 0.16 (5) 4.3E-3
IL3 6.72 ± 0.14 (5) 1.7E-5
S4 6.98 ± 0.17 (5) 1.7E-5