Flowchart of Ebbie. Indicated are Ebbie's functions and the cgi-scripts required for each. Ebbie's front page is created by index.html. The database information is submitted to Ebbie's main page, created by index.cgi. Index.cgi can update the currently used cloning primers (5-CP and 3-CP). From the main page, several scripts can be called. DNA sequencing text files are uploaded and analyzed by ebbie.cgi. Depending on the number of inserts found, annoch.cgi is called to deposit the insert into the MySQL database. If multiple inserts are found, ebbie2.cgi and annoch2.cgi are used for the same purpose. From Ebbie's main page a range of database review/manipulation tools can be accessed: Logbook (table created by logbook.cgi), View All, Lost & Found (tables created by viewall.cgi) and Annotation Change (also using annoch.cgi to update database entry).