Example MACSIMS alignment analysis presented in the JalView applet. A. Overview of complete alignment. Regions calculated by MACSIMS are coloured according to their phylogenetic distribution. The red box indicates the section of the alignment shown in B and C. A conservation score  for each alignment column is shown below the alignment. B. Detailed view of one part of the alignment. Metal binding and active site residues are indicated, together with Prosite motifs. Mutated residues are shown with a pink background. C. The same part of the alignment as in B, with secondary structure elements highlighted.