Table 1 Coverage and amplicon characteristics for the adjacent Weisburg and Lane universal primer sets in 47,257 bacterial sequences.
Forward Primer | Reverse Primer | Occurrence of Primer Pair in All Bacterial Sequences | Occurrence of Primer Pair in Enteric Sequences | Average Amplicon Length and Std. Dev. (All Bacteria) | Average Amplicon Length and Std. Dev. (Enterics only) |
|---|---|---|---|---|---|
"Weisburg FWD" AGAGTTTGATCCTGGCTCAG AGAGTTTGATCATGGCTCAG AGAGTTTGATCCTGGCTTAG AGAATTTGATCTTGGTTCAG | "Lane A" CAGCAGCCGCGGTAATAC CAGCAGCCGCGGTAATTC CAGCCGCCGCGGTAATAC CAGCCGCCGCGGTAATTC | 9337/47257 = 19.76% | 333/1655 = 20.12% | 518.78 ± 37.94 bp | 530.42 ± 3.38 bp |
"Lane A" CAGCAGCCGCGGTAATAC CAGCAGCCGCGGTAATTC CAGCCGCCGCGGTAATAC CAGCCGCCGCGGTAATTC | "Lane B" AAACTCAAAGGAATTGACGG AAACTCAAATGAATTGACGG AAACTTAAAGGAATTGACGG AAACTTAAATGAATTGACGG | 38310/47257 = 81.07% | 1553/1655 = 93.84% | 408.83 ± 7.35 bp | 409.17 ± 2.40 bp |
"Lane B" AAACTCAAAGGAATTGACGG AAACTCAAATGAATTGACGG AAACTTAAAGGAATTGACGG AAACTTAAATGAATTGACGG | "Lane C" GTACACACCGCCCGT GCACACACCGCCCGT | 36,058/47,257 = 76.30% | 1401/1655 = 84.65% | 500.83 ± 13.94 bp | 501.26 ± 1.06 bp |
"Lane C" GTACACACCGCCCGT GCACACACCGCCCGT | "Weisburg REV" GGTTGGATCACCTCCTTA AAGTCGTAACAAGGTAACCGT AAGTCGTAACAAGGTAGCCGT AAGTCGTAACAAGGTATCCGT | 9991/42257 = 21.14% | 373/1655 = 22.54% | 123.62 ± 57.24 bp | 122.24 ± 8.1978 bp |