Figure 6

Dot plots of the values for the various validation indices for technical and biological validation samples. Dot plots of (A) slope, (B) y-intercept, (C) precision (Pearson r), (D) accuracy (E) concordance correlation coefficient (CCC) and (F) intraclass correlation coefficient (ICC). In each dot plot, the dots represent the comparisons between technical (open dots) and biological replicates (filled dots) of microarray to qrPCR experiments. As with the simulated data, the slope and y-intercept results for the top-ranked comparisons are unbiased relative to random-stratified comparisons, but they are more variable. The precision, accuracy, CCC and ICC estimates are, similar to the simulated data, more variable and downwardly biased.