Evaluation of ProFAT using the domain families PABP, PLAT and HNF-1α. (A) Positive identification of PABP, PLAT and HNF-1α domain family members using HMMerThread and the Annotation Engine. Based on the Superfamily database , all members of the PABP, PLAT and HNF-1α family were subjected to high-throughput HMMerThread and Annotation Engine searches. Results show the percentage positive identification of family members using these two different pipelines, as well as the domain search programs HMMer and RPS-BLAST. (B) Keyword-positive hits of PABP, PLAT and HNF-1α domain family members in ProFAT's Annotation Engine. Results show the frequency of keywords identified within the different keyword lists used. Abbreviations used in (A): AE: Annotation Engine.