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Figure 3 | BMC Bioinformatics

Figure 3

From: Normalization and expression changes in predefined sets of proteins using 2D gel electrophoresis: A proteomic study of L-DOPA induced dyskinesia in an animal model of Parkinson's disease using DIGE

Figure 3

2D-M plot for gel 4 and 12. Spatial gel plots for gel 4 and 12 (A) before, and (B-E) after normalization using different methods. A reconstructed gel image with increasing isoelectric point (pI) (left-right) and decreasing protein mass (top-down). For method A, B, E, F and G the M-values are calculated as log2Cy5/Cy3 and for method C and D the M-values are calculated as log2(Cy5/Cy2)/(Cy3/Cy2). The ten percent highest and lowest M-values in each gel are color coded red and blue respectively. For several of the normalization methods the spatial bias remains.

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BMC Bioinformatics

ISSN: 1471-2105

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