Statistical analysis of an RNA titration series evaluates microarray precision and sensitivity on a whole-array basis

Table 2 Standard deviation with which a typical log-fold change is measured using a minimal experiment.

The table gives the median standard deviation across probes together with the first and third quartiles. For the two-color platforms the design may be direct using one array or indirect via a common reference using two arrays. For Affymetrix, two arrays are used. The two-color calculations assume that estimates of probe-specific dye effects are available, .e.g., from a larger experiment including dye-swaps.

ISSN: 1471-2105