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Table 3 The comparison of four approaches. The table listed ΔΔCt, standard error, P-value and confidence interval derived from the four methods presented in the article. Neither SAS package nor the macro used provides the standard error for Wilcoxon two group test. We consider confidence interval to be sufficient for further data transformation.

From: Statistical analysis of real-time PCR data

Model ΔΔCt Standard Error P-Value Confidence Interval
Multiple Regression -0.6848 0.1185 < 0.0001 (-0.4435, -0.9262)
ANCOVA -0.6848 0.1185 < 0.0001 (-0.4435, -0.9262)
t-test -0.6848 0.1303 < 0.0001 (-0.4147, -0.955)
Wilcoxon Test -0.6354   < 0.0001 (-0.4227, -0.8805)