Figure 12
From: Nanopore Detector based analysis of single-molecule conformational kinetics and binding interactions
The determination of aptamers can be done (or initiated) via Systematic Evolution of Ligands by Exponential Enrichment (SELEX), as shown schematically on the left. What is proposed here is a linkage to a na nopore-detector dir ected (NADIR) search for aptamers that is based on bound-state lifetime measurements. NADIR complements and augments SELEX in usage: SELEX can be used to obtain a functional aptamer, and NADIR used for directed modifications (for stronger binding affinity, for example).