Figure 2

Sample query output and graph representation from SPIDer. (A) Partial results of inter-member interactions from the query for the SCF-CDC4 complex (search by inputting the MIPS identifier '445.10'). The table of cross-member interactions has the following information: the information for two interacting proteins, their RSS values on the three ontologies (CC, BP and MF), the cross references to other binary interaction datasets (DIP, BIND and MIPS physical interactions) and the MIPS complexes, as well as the hyperlinks to the evidence information. This table is designed to be clickable and provides hyperlinks of the proteins to SGD. Both DIP binary interactions and MIPS complexes have links to their home pages. (B) A graphical representation of the inter-member interaction network of the SCF-CDC4 complex using the default score cutoff. Nodes and edges represent proteins and cross-member interactions, respectively. Each node is labeled standard gene name. Each edge is depicted in colour representing the RSS score, which is the minimum of the BP and CC RSS values. In addition, the ORF name and primary SGD identifier are obtained when the cursor is positioned over a node, and score value over an edge (an example of the interaction between CDC34 and SKP1 is shown). Five different scores of edges in the legend are depicted in five colours.