Figure 1
From: MicroTar: predicting microRNA targets from RNA duplexes
MicroTar algorithm. Beginning with a set of fasta-formatted query (miRNA) sequences and target (mRNA) sequences, the MicroTar algorithm predicts the minimum free energy of the each mRNA molecule, searches for seed sites, and performs a constrained fold where each seed match is, in turn, bound in the miRNA-mRNA heterodimer; the output is a list of putative duplexes more stable than free mRNA, along with images of bound and unbound mRNA secondary structure. This result is subsequently subjected to a statistical analysis to determine the significance of each miRNA-mRNA match.