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Table 5 Precision of each calculation model

From: Statistical significance of quantitative PCR

Model Slope SD p-value r2
Sigmoid 0.085 1.225 4.4E-12 0.00004
LR N0 1.830 0.621 4.7E-26 0.07441
Exponential 0.707 1.047 0.004 0.00421
ECt 1.103 0.127 1.2E-13 0.40965
ΔCt 0.689 0.013 1.4E-149 0.96041
(Pavrg E)Ct 0.994 0.026 0.021 0.93213
(Savrg E)Ct 0.996 0.027 0.170 0.92419
  1. Expected and measured relative concentrations were plotted and a linear regression was performed (Additional File 3). The slopes values were submitted to a t-test to evaluate if they are statistically different from 1, which would indicate a poor correlation between expected and measured concentrations. A p-values smaller than 0.05 indicate that the slope is significantly different from 1. Correlation coefficient (r2) values indicate the width of the spreading of individual data around the linear regression line. Higher r2 indicate more robust models.