Table 1 Empirical and statistical scores along with associated parameters (p 1 and p 2) for each spectrum shown in Figure 1. The data were obtained for mass accuracies of 1.0 Da, 0.1 Da and 0.01 Da. Confidence levels were calculated based on a search space of 2726345 theoretical peptides. The confidence levels for the pp and pp2 scores are denotes as confidence level and confidence level2
Mass accuracy | Spectrum | p 1 | p 2 | score | pp | pp2 | Confidence level | Confidence level 2 |
|---|---|---|---|---|---|---|---|---|
0.01 Da | a | 2.1. × 10-5 | 7.9. × 10-4 | 69 | 53.8 | 307.6 | 52.0 | 305.8 |
|  | b | 2.1. × 10-5 | 2.1. × 10-3 | 75 | 69.0 | 307.6 | 67.2 | 305.8 |
|  | c | 2.1. × 10-5 | 9.2. × 10-4 | 59 | 43.0 | 307.6 | 41.2 | 305.8 |
|  | d | 2.1. × 10-5 | 1.5. × 10-3 | 19 | 75.2 | 307.6 | 73.4 | 305.8 |
|  | e | 2.1. × 10-5 | 6.5. × 10-4 | 27 | 36.6 | 307.6 | 34.8 | 305.8 |
0.1 Da | a | 2.1. × 10-4 | 7.8. × 10-3 | 77 | 38.6 | 203.9 | 35.8 | 201.1 |
|  | b | 2.1. × 10-4 | 2.1. × 10-2 | 95 | 46.7 | 157.5 | 43.9 | 154.7 |
|  | c | 2.1. × 10-4 | 9.1. × 10-3 | 68 | 26.9 | 274.7 | 24.1 | 271.9 |
|  | d | 2.1. × 10-4 | 1.5. × 10-2 | 20 | 45.2 | 37.3 | 42.4 | 34.5 |
|  | e | 2.1. × 10-4 | 6.5. × 10-3 | 28 | 23.7 | 82.8 | 20.9 | 80.0 |
1.0 Da | a | 2.1. × 10-3 | 7.8. × 10-2 | 100 | 22.1 | 21.3 | 18.3 | 17.5 |
|  | b | 2.1. × 10-3 | 2.1. × 10-1 | 120 | 16.0 | 12.1 | 12.2 | 8.3 |
|  | c | 2.1. × 10-3 | 9.1. × 10-2 | 74 | 7.8 | 23.1 | 4.0 | 19.3 |
|  | d | 2.1. × 10-3 | 1.5. × 10-1 | 55 | 15.5 | 6.1 | 11.7 | 2.3 |
|  | e | 2.1. × 10-3 | 6.4. × 10-2 | 36 | 14.7 | 9.0 | 10.9 | 5.2 |