Table 3 BLAST scores of added reactions in S. cerevisiae

methionine γ-lyase

1e-58,1e-68

Cystathionine gamma-lyase

Pseudomonas putida

2-hydroxyglutarate synthase(no gene identified yet)

----------

---------------

E. coli

2-oxoglutarate reductase/phosphoglycerate dehydrogenase

2e-14, 3e-18

3-phosphoglycerate dehydrogenase

Bacillus subtilis

deoxyribose-phosphate aldolase

7.7, 0.006

Phosphatidylinositol 3-kinase TOR2

Mycoplasma pneumoniae

D-lactate dehydrogenase

2, 3e-04

Protein of unknown function

E. coli

L-tryptophan aminotransferase

no information

no information

Enterobacter cloacae

Methylglyoxal synthase

1.5, 0.001

carbamyl phosphate synthetase

E. coli

phenylpyruvate decarboxylase

3E-35, 1e-39

Chain A, Pyruvate Decarboyxlase

Azoarcus sp. EbN1

tagatose-1,6-bisphosphate aldolase 2

1e-12, 1e-16

Fructose 1,6-bisphosphate aldolase

E. coli

tryptophan 2-monooxygenase

2.2, 5e-05

orf:PZA105

E. coli

5,10-methylenetetrahydrofolate reductase(present in the cytosol)

1E-38, 2e-42

Isozyme of methylenetetrahydrofolate reductase

E. coli

adenine phosphoribosyltransferase(present in cytosol)

2E-25, 1e-29

Adenine phosphoribosyltransferase

E. coli

guanylate kinase(present in cytosol)

9E-38, 7e-42

Guanylate kinase

E. coli

putative NAD+ kinase(present in cytosol)

2E-27, 6e-28

ATP-NADH kinase

E. coli

adenylate kinase(present in cytosol and mitochondria)

7E-51, 5e-55

Adenylate kinase

E. coli