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Figure 5 | BMC Bioinformatics

Figure 5

From: Impact of image segmentation on high-content screening data quality for SK-BR-3 cells

Figure 5

Variability in the well- and poorly-segmented populations. The absolute value of the coefficient of variation (ACV) was computed for each of the 116 morphological features, within well- and poorly-segmented populations from a representative set of DMSO-vehicle treated cultures. Each population contained approximately 3300–4000 cells. Features were sorted by their ACV in the well-segmented population for display purposes. The red line labelled "w" indicates ACV for features in the well-segmented population; the black line labelled "p" is ACV of the same features in the poorly segmented population. The asterisks indicate the 7 features that were used in the SVM-RBF classifier. The overall similarity in ACV between the two populations indicates that the well-segmented population retained significant biological variation of interest.

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