Number of oligonucleotides outside of the quality control criteria ploted along variable weight sets. The theoretical microarray oligonucleotide sets for the P. falciparum genome were designed using 162 weight sets assembled from intervals of uniqueness weights (WBLAST), GC content weights (WGC) self-binding weights (WSW), and sequence complexity weights (WLZ). In these number of oligonucleotides rejected based on the 40%-uniqueness rule (> 40% continuous sequence identity to a second target) was determined and plotted for each weight set. Each curve represents a profile of rejected oligonucleotide numbers with increasing uniqueness weights while the other weights are kept constant (A). Similar to the uniqueness weight profiles number of oligonucleotides rejected using the GC content range rule (GC content outside of the 31.4% ± 5%) were plotted with the increased CG content weights (B).